Application of flow cytommetry detection of platelet associated antibody in patients with immune thrombocytopenic pupura
10.3969/j.issn.1673-4130.2014.13.010
- VernacularTitle:流式细胞术检测血小板相关抗体在免疫性血小板减少症中的应用
- Author:
Na REN
;
Guangbin QIU
;
Chengbin WANG
- Publication Type:Journal Article
- Keywords:
flow cytometry;
blood platelets;
purpura,thrombocytopenic,idiopathic
- From:
International Journal of Laboratory Medicine
2014;(13):1689-1690,1693
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of the flow cytometry(FCM)for detecting platelet associated antibody in the di-agnosis of immune thrombocytopenia(ITP).Methods Platelet associated antibody,expression rate and the fluorescence intensity of platelet-associated immunoglobulin(PAIg)were measured in 51 patients with ITP(23 cases of newly diagnosed ITP and 28 cases of persistent ITP),21 patients with non-ITP and 60 healthy individuals.The correlation between the detection results with the platelet and megakaryocyte counts was performed;the expression rates of PAIg before and after treatment were compared.Results The fluorescence positive percentage and mean fluorescence intensity of IgG,Ig A and IgM in the patients group were significantly high-er than those in the control group with statistical difference(P <0.01).Compared with the non-ITP group,the difference had statis-tical significance(P <0.05).Compared with the autoimmune disease group,the difference had no statistical significance(P >0.05);the difference between the autoimmune disease group and the healthy control group had statistical significance(P <0.01 );in the persistent ITP group,PAIg in the complete response group had statistical difference between the groups before treament.PAIg in the response group also had statistical difference between the groups before treament (P <0.05).Conclusion FCM for detecting PAIg can be used in the diagnosis,differential diagnosis and the disease condition monitoring of ITP.
