Expression,purification and biological activity assays of global human C1 q and tumor necrosis factor related protein-2
10.3969/j.issn.1001-1978.2014.07.029
- VernacularTitle:C1 q／T NFα相关蛋白-2球状功能区的表达、纯化及活性分析
- Author:
Hongbo LI
;
Xing HU
;
Na LI
;
Donghai WU
- Publication Type:Journal Article
- Keywords:
C1 q and tumor necrosis factor related protein-2;
global domain;
Escherichia coli;
recombinant protein;
affinity purification;
gel filtration chromatography
- From:
Chinese Pharmacological Bulletin
2014;(7):1023-1025,1026
- CountryChina
- Language:Chinese
-
Abstract:
Aim To prepare soluble global human C1 q and tumor necrosis factor related protein-2 in Escherichia coli. Methods Recombinant expression plasmid was transformed into strain BL21-codonplus (DE3),and the recombinant protein of Trx-gH2 was expressed by IPTG induction and then purified by Ni-NTA affinity and gel filtration chromatography.Results The purified recombinant Trx-gH2 was shown to be active under in vi-vo and in vitro assay conditions.Conclusion Active recombi-nant global hCTRP2 is efficiently prepared from Escherichia coli protein expression system.
