Effects of Calcium Gluconate, a Water Soluble Calcium Salt on the Collagen-Induced DBA/1J Mice Rheumatoid Arthritis.
- Author:
Ki Cheul SOHN
1
;
Su Jin KANG
;
Joo Wan KIM
;
Ki Young KIM
;
Sae Kwang KU
;
Young Joon LEE
Author Information
1. Department of Preventive Medicine, School of Medicine, Catholic University of Daegu, Daegu 705-718, Repulic of Korea.
- Publication Type:Original Article
- Keywords:
Calcium gluconate;
Rheumatoid arthritis;
Anti-inflammation;
Anti-oxidation;
Immunomodulation
- MeSH:
Animals;
Arthritis;
Arthritis, Rheumatoid*;
Body Weight;
Calcium Gluconate*;
Calcium*;
Cartilage;
Collagen;
Humans;
Immunization;
Immunomodulation;
Interleukin-6;
Knee;
Knee Joint;
Lymph Nodes;
Male;
Malondialdehyde;
Mice*;
Mice, Inbred DBA;
Oxidative Stress;
Peroxidase;
Spleen;
Tumor Necrosis Factor-alpha;
Water*
- From:Biomolecules & Therapeutics
2013;21(4):290-298
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study examined the effects of calcium (Ca) gluconate on collagen-induced DBA mouse rheumatoid arthritis (CIA). A single daily dose of 200, 100 or 50 mg/kg Ca gluconate was administered orally to male DBA/1J mice for 40 days after initial collagen immunization. To ascertain the effects administering the collagen booster, CIA-related features (including body weight, poly-arthritis, knee and paw thickness, and paw weight increase) were measured from histopathological changes in the spleen, left popliteal lymph node, third digit and the knee joint regions. CIA-related bone and cartilage damage improved significantly in the Ca gluconate- administered CIA mice. Additionally, myeloperoxidase (MPO) levels in the paw were reduced in Ca gluconate-treated CIA mice compared to CIA control groups. The level of malondialdehyde (MDA), an indicator of oxidative stress, decreased in a dose-dependent manner in the Ca gluconate group. Finally, the production of IL-6 and TNF-alpha, involved in rheumatoid arthritis pathogenesis, were suppressed by treatment with Ca gluconate. Taken together, these results suggest that Ca gluconate is a promising candidate anti-rheumatoid arthritis agent, exerting anti-inflammatory, anti-oxidative and immunomodulatory effects in CIA mice.
