Role of CREB in LPS-induced injury of RPMVEC
10.3969/j.issn.1001-1978.2014.07.018
- VernacularTitle:cAMP反应元件结合蛋白在LPS致肺微血管内皮细胞损伤过程中的作用
- Author:
Xiujuan XU
;
Gengyun SUN
;
Qinghai YOU
;
Dan ZHANG
- Publication Type:Journal Article
- Keywords:
cAMP response element binding protein;
protein kinase A;
lipopolysaccharide;
pulmonary micro-vascular endothelial cell;
rat;
permeability;
acute re-spiratory distress syndrome
- From:
Chinese Pharmacological Bulletin
2014;(7):965-968,969
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of cAMP re-sponse element binding protein (CREB)in the injury of rat pulmonary microvascular endothelial cell (RPM-VEC)induced by LPS.Methods RPMVECs were i-solated and cultured in vitro,Western-blot was used to assay phosphorylation levels of CREB.Endothelial per-meability was determined by measuring the influx of Evans blue-labeled albumin across endothelial mono-layer.Results LPS increased CREB phosphorylation at Ser 1 3 3 in RPMVEC in a time-dependent manner , peaked at 30 min,but still higher at 120 min compared with basal control group.Pretreatment of cells with PKA inhibitor V5681 nearly suppressed the CREB phosphorylation stimulated in the presence of LPS,and the monolayer permeability of PMVEC was significantly increased. Conclusions LPS rapidly induces the phosphorylation of CREB in RPMVEC,and PKA me-diates the process.During the process of LPS-stimula-ted injury of RPMVEC,phosphorylation of CREB may play a protective role.
