Expression of angiogenic factors in myocardial tissue of diabetic rats
10.3969/j.issn.1000-4718.2014.06.024
- VernacularTitle:血管生成因子在糖尿病大鼠心肌组织的表达
- Author:
Linlin CHEN
;
Chun GUI
;
Xiaomin WEI
;
Liquan CHEN
;
Liguang ZHU
- Publication Type:Journal Article
- Keywords:
Vascular endothelial growth factor;
Angiopoietins;
Endostatin;
Diabetic cardiomyopathies;
Rats
- From:
Chinese Journal of Pathophysiology
2014;(6):1098-1102,1109
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the expression of angiogenesis factors in the myocardial tissue of streptozotocin-induced diabetic rats .METHODS:The diabetic rat model was induced by intraperitoneal injection of streptozotocin .After 12 weeks, the cardiac function was measured by MPA cardiac function analysis system .The myocardial collagen volume fraction ( CVF) was assessed by Masson staining .The capillary vessels was quantified as the ratio of capillary to myocyte (C/M) using CD31 immunostaining.The expression levels of vascular endothelial growth factor (VEGF), angiopoietin ( Ang)-1, endostatin and Ang-2 were observed by Western blotting .RESULTS:Compared with normal control group , the left ventricular end-diastolic pressure (LVEDP) was evidently increased (P<0.01), but left ventricular pressure rise maximum rate (+dp/dtmax), left ventricular pressure decrease maximum rate (-dp/dtmax) and the ratio of capillary/myo-cyte (C/M) were significantly decreased (P<0.05).The CVF and the expression level of endostatin were significantly increased, whereas the expression levels of VEGF and Ang-1 evidently decreased (both P<0.05) in diabetic rats.Howev-er, no marked difference in the expression of Ang-2 between the 2 groups was observed (P>0.05).CONCLUSION:Im-balances between the angiogenic factors (VEGF and Ang-1) and anti-angiogenic factors (endostatin) may play an impor-tant role in the pathogenesis of diabetic cardiomyopathy .
