The study on organ weights, blood physiological and biochemical parameters and immune cells in BALB/cA-nu mice
10.3969.j.issn.1671.7856.2014.005.009
- VernacularTitle:BALB／cA-nu 小鼠主要脏器参数、血生理生化指标和免疫细胞的测定
- Author:
Xuying WU
;
Yushu TIAN
;
Jingfeng WU
;
Weibin ZHENG
;
Yu WANG
;
Feifei WANG
;
Wenming ZHANG
;
Zhiwei YANG
- Publication Type:Journal Article
- Keywords:
BALB/cA-nu mice;
Organ weights;
Blood physiological and biochemical parameters;
Immune cells
- From:
Chinese Journal of Comparative Medicine
2014;(5):42-46,41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure the organ weights , blood physiological and biochemical parameters , and immune cells of BALB/cA-nu mice .Methods BALB/cA-nu mice at five and ten weeks of age were selected , and the organ weights , blood physiological and biochemical parameters were observed .The percentages of CD 3+, CD4+, CD8+, CD19+, B220+, NK1.1+, and CD11b+were detected by FCM in BALB/cA-nu mice at six weeks of age in terms of its T, B lymphocyte cells and NK cell activity .Results At the same age, the weights of the body , liver, and kidney in male mice are significantly higher than females .The HGB、MCH、HCT in male mice are significantly higher than females (P <0.05).The weights of the lung, liver, and kidney, and the parameters of TP、GLOB、CHO、TBIL、UN are significantly lower at five weeks of age comparing with ten weeks (P <0.05).The BALB/cA-nu mice lacks T cells(0.18 ±0.06)%、CD4+T cells(0.26 ±0.08)%、CD8+T cells(0.13 ±0.04)%、The percentage of B cells is CD19+B cells (30.10 ±2.74)% and B220 +B cells(30.55 ±2.77)%.The percentage of NK cells is (1.35 ±0.29)%, and the percentage of granulocytes is (47.90 ±5.48)%.The BALB/cA-nu mice lack T cells and the group of cells .Conclusion The study suggests that the organ weights , blood physiological and biochemical parameters are affected by age and gender in BALB/cA-nu mice that lack T cells immune function .The studied parameters of BALB/cA-nu mice are similar with the same strain in other countries .
