Biomarkers of hyperlipidemia cholesterol metabolism in hamster
10.3969/j.issn.1001-1978.2014.06.030
- VernacularTitle:金黄地鼠高脂血症模型胆固醇代谢紊乱的生物标志物的研究
- Author:
Zhuoying KANG
;
Xinxin CHU
;
Runmei YANG
;
Min JI
;
Ying YU
;
Nannan GAO
- Publication Type:Journal Article
- Keywords:
hamster;
hyperlipidemia;
LDL-R;
SREBP-2;
CYP7A1;
FXR;
LXRα
- From:
Chinese Pharmacological Bulletin
2014;(6):880-882,883
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish the hyperlipidemic model and ex-plore the mechanism of hypercholesterolemia in hamster. Meth-ods Hamsters were randomly divided into the control and model groups. The hamsters in the control group were fed with the standard chow and the model group were fed with the high fat di-et. Serum lipids and CYP7A1 activity were detected by enzymat-ic method. The molecular mechanism of cholesterol metabolism was investigated by real-time PCR. Results In comparison with the control group, the concentrations of serum TC, LDL-C, TG and hepatic TC, TG were significantly increased in the model
group. The mechanism research showed that in hamsters fed with the high fat diet, the CYP7A1 activity and the mRNA expres-sions of hepatic LDL-R, SREBP-2, CYP7A1, LXRαwere down-regulated, the expression of hepatic FXR was up-regulated. Conclusion The hyperlipidemic model could be developed in hamsters fed with the high fat diet for 4 weeks. LDL-R, SREBP-2, CYP7A1, FXR and LXRαare the biomarkers of hypercholes-terolemia, and also the targets of hypolipidemic drugs.
