Effect of baicalin on proliferation and migration activity in human skin SCC cells

VernacularTitle:黄芩苷对皮肤鳞癌细胞生长和迁移能力的影响
Author: Ziliang YANG ; Dan LUO ; Bingjiang LIN ; Qihong QIAN ; Xiuqin YU ; Miaomiao WANG ; Wei MIN
Publication Type:Journal Article
Keywords: baicalin; cofilin-1; skin squamous cancer A431 cell; siRNA; cell proliferation; cell migration
From: Chinese Pharmacological Bulletin 2014;(6):821-824,825
CountryChina
Language:Chinese
Abstract: Aim To investigate the effect of baicalin on cell proliferation and cell migration in human skin SCC A431 cell line. Methods The A431 cells were incu-
bated with 50 mg·L-1 baicalin. The protein level of cofilin-1 was assayed by Western blot. Cofilin-1 specific siRNA fragment was designed , synthesized and trans-
fected into A431 cells. The proliferative activity and migration ability of cells were assessed by CCK8 assay and scratch wound healing assay separately. ResultsWestern blot results showed that baicalin treatment in-hibited the cofilin-1 protein expression to 49.3% com-pared with the control group. Single baicalin treatment and cofilin-1 silencing could drease the A431 cell growth and migration. And cofilin-1 silencing signifi-
cantly enhanced the efficacy of baicalin. Conclusions Baicalin could significantly inhibit the tumor cell's growth and migration in the A431 cell line. And cofi-lin-1 might become the potential target gene to enhance the effect of anticancer drugs.