Effect of PDTC on MCP-1 in rejection of cardiac allograft
10.3760/cma.j.issn.1001-4497.2014.05.013
- VernacularTitle:PDTC对大鼠心脏移植排斥反应中MCP-1表达的干预
- Author:
Xiao BAI
;
Jian ZHANG
;
Guangmin SONG
;
Xin ZHAO
;
Wei WANG
- Publication Type:Journal Article
- Keywords:
Heart transplantation;
Graft rejection;
Pyrrolidine dithiocarbamate;
Monocyte chemotactic protein-1;
Nuclear factor of kappa B
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2014;30(5):304-306,308
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of pyrrolidine dithiocarbamate(PDTC) on monocyte chemotactic protein1 (MCP-1) in rejection of cardiac allograft and its mechanisms.Methods Heterotopic cervical heart transplantation was performed by cuff-technique.The SD rat recipients were randomly divided into 3 groups:AR group (Acute rejection,n =12),both the recipients and donors were without any treatment.CsA group(n =12),the recipients were treated with 10 mg/kg cyclosporine A after transplantation.PDTC group(n =12),the recipients were treated with 100 mg/kg PDTC after transplantation.All the cardiac allografts were harvested at different time post transplantation according to requirements.We studied allograft myocardial fibrosis wih the help of Masson stain,immuno-histo-chemistry and western blot also were used to detect the expression of MCP-1.Results The survival time of the cardiac allografts was significantly longer in PDTC group than in acute rejection group and CsA group(P ＜ 0.01),and myocardial fibrosis of cardiac allografts in PDTC group was significantly decreased (P ＜ 0.01).The IOD in PDTC group was markedly lower than in CsA group (P ＜ 0.01).Conclusion As the inhibitor of NF-κB,PDTC can significantly relieve rejection of cardiac allograft by inhibiting the expression of MCp-1.
