Activation of protease-activated receptor 2 inhibits apoptosis of lung cancer cells
10.3969/j.issn.1001-1978.2014.05.021
- VernacularTitle:激活蛋白酶活化受体2抑制肺癌细胞凋亡
- Author:
Shaohong HUANG
;
Jun AN
;
Yun LI
;
Junhang ZHANG
;
Jian RONG
- Publication Type:Journal Article
- Keywords:
epidermal growth factor receptor;
prote-ase-activated receptor 2;
lung cancer;
apoptosis;
Bax;
Bcl-xL
- From:
Chinese Pharmacological Bulletin
2014;(5):684-687,688
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the activation of prote-ase-activated receptor 2 ( PAR2 ) in regulation of the expression of epidermal growth factor receptor ( EGFR) and apoptosis of lung cancer ( LC) cells. Methods LC cells A549 and its EGFR-silenced counterpart were incubated in the medium added with tryptase. Quanti-tative RT-PCR and Western blotting were used to de-tect the expression of EGFR in A 5 4 9 cells . The apop-tosis and Bax/Bcl-xL of cells were also recorded. Re-sults Treating A549 cells with tryptase in the culture for 48 hrs resulted in a marked increase in the expres-sion of EGFR in A549 cells. Marked decreases in a tryptase dose-dependent manner in apoptotic A549 cells were detected in the presence of tryptase. Expo-sure to tryptase markedly decreased the levels of Bax and increased the levels of Bcl-xL in A549 cells, which were not shown in EGFR-deficient A549 cells. Conclusion Tryptase can increase the expression of EGFR in LC cell line, A549 cells, which protects A549 cells from apoptosis, increases Bcl-xL, and sup-presses Bax in A549 cells.
