Effect of IL-29 on trypsin-induced protease-activated receptor expression on P815 mast cells
10.3969/j.issn.1000-484X.2014.05.008
- VernacularTitle:IL-29对胰蛋白酶诱导的肥大细胞PARs表达的调节作用
- Author:
Li SUI
;
Dong CHEN
;
Huiyun ZHANG
;
Shaoheng HE
- Publication Type:Journal Article
- Keywords:
Mast cell;
Interleukin-29(IL-29);
Trypsin;
Protease activated receptors (PARs);
Flow cytometry
- From:
Chinese Journal of Immunology
2014;(5):609-612,622
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the modulatory effect of IL-29 on trypsin-induced protease activated receptors (PARs) ex-pression on P815 mast cell.Methods:After P815 mast cells were challenged with different concentrations of IL-29 alone or combined with trypsin for 2 h, 6 h and 16 h, the challenged cells were collected and analysed by flow cytometry to detect the protein expression of PARs on P815 cells, and analysed by real time RT-PCR to detect the mRNA expression of PARs on P 815 cells.Results:Compared with the corresponding control , IL-29 induced significantly decreased expression of PAR-1 at protein and mRNA level on P815 cells, and upregulated PAR-3, PAR-4 mRNA level on P815 cells, whereas IL-29 did little effect on the expressions of PAR-2,3,4 at protein level on P815 cells accordingly.Preincubation of mast cell with IL-29 did not alter trypsin-induced PAR-1 expression on P815 cells, whereas up-regulated expression of PAR-2, 3, 4 were detected when P815 cell were pre-treated with IL-29 before being challenged with trypsin compared with the corresponding control .Conclusion: IL-29 can upregulate trypsin-induced PARs expression on mast cells through which participated in mast cell related inflammation .
