Akt phosphorylation and mechanism of TRAIL-resistance in A549 cells
10.3969/j.issn.1000-484X.2014.05.013
- VernacularTitle:Akt磷酸化在A549细胞抵抗rsTRAIL蛋白诱导细胞凋亡的研究
- Author:
Lei LIU
;
Yanqiu FANG
;
Yaling QI
;
Xiaodan LU
;
Hanfeng WEI
;
Yan TAN
- Publication Type:Journal Article
- Keywords:
A549 cells;
rsTRAIL protein;
Akt;
Perifosine
- From:
Chinese Journal of Immunology
2014;(5):630-632,638
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate mechanism of TRAIL-resistance in A549 cells ( a cell line of non-small cell lung carcino-ma cells) due to Akt phosphorylation .Methods:A549 cells were treated with Akt inhibitor Perifosine and rsTRAIL protein individual-ly and in combination.The expressions of Akt phosphorylation(p-Akt),c-FLIPLL and caspase-8 were detected by Western blot.The apoptotic rate of the A549 cells treated was detected by flow cytometry and the cell proliferation was evaluated by MTT assay .Results:A549 cells showed the increased level of Akt phosphorylation mediated by rsTRAIL protein .Treatment with the Akt inhibitor Perifosine induced a suppression of Akt activation in A 549 cells and a concomitant decrease in the expression of c-FLIPLL .As a result, Perifosine significantly enhanced TRAIL-induced apoptosis rate of (76.5 ±3.02)%and cytotoxic rate of (83.2 ±2.54)%by promoting the activ-ity of caspase-8.Conclusion:Akt activity promotes A549cells survival against TRAIL-induced apoptosis and that the cytotoxic effect of rsTRAIL protein can be enhanced by modulating the Akt phosphorylation in human non -small cell lung carcinoma cells .
