Near infrared heptamethine cyanine dye IR-783-mediated tumor imaging
10.3969/j.issn.1005-4847.2014.02.004
- VernacularTitle:近红外荧光染料 IR-783介导的肿瘤成像
- Author:
Caiqin ZHANG
;
Hai ZHANG
;
Yong ZHAO
;
Fengfeng MAO
;
Bing BAI
;
Changhong SHI
- Publication Type:Journal Article
- Keywords:
Heptamethine cyanine IR-783;
Near infrared fluorescence;
Tumor imaging;
mouse;
Dog
- From:
Acta Laboratorium Animalis Scientia Sinica
2014;(2):17-19,56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of near infrared heptamethine cyanine dye IR-783-mediated specific tumor imaging in spontaneous tumor of dogs .Methods IR-783 was intraperitoneally injected to nude mice models of transplanted tumor in a dose of 5μmol/kg.The metabolic time course of IR-783 was detected by in vivo imager .Based on the results of above observation , IR-783 was injected to dogs with spontaneous tumor in a dose of 1.5μmol/kg.The site of intravenous injection was the hind leg .Tumor and peri-tumoral tissues were removed at 5 days after IR-783-injection for fluorescence imaging , pathology and frozen section fluorescence examinations .Results After i.p.IR-783 injection to nude mice models of transplanted tumor , the transplanted tumor tissues of nude mice had stronger specific fluorescence than normal tissues by imaging at 8 days after injection .After i.v.IR-783 injection to four dogs with spontaneous tumor , the fluorescence signal in the tumor tissues was stronger than that in the normal tissues at 5 days after injection .Conclusions Near infrared fluorescent dye IR-783 could be specifically taken up by tumor tissues , and can be used for specific diagnosis of tumor.It has an important clinical application prospect .
