Selenium dioxide inducing apoptosis of cervical cancer cells by regulating apoptosis-related proteins
10.3969/j.issn.1671-8348.2014.10.023
- VernacularTitle:二氧化硒调控凋亡相关蛋白诱导宫颈癌细胞凋亡的影响
- Author:
Sisun LIU
;
Jieqi XIONG
;
Qinghua MIN
;
Ling GUO
;
Min XIU
;
Feng HE
;
Yuanlei LOU
;
Fei GUO
- Publication Type:Journal Article
- Keywords:
selenium dioxide;
apoptosis;
caspase-3;
gene,p53
- From:
Chongqing Medicine
2014;(10):1218-1220,1223
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inducing effects of selenium dioxide(SeO2 ) on the apoptosis in human cervical carcino-ma cell line Hela and its influence on the expression of apoptosis-related proteins caspase-3 and P53 .Methods Hela cells were trea-ted with different concentrations of SeO2 for 24 h in vitro ;the morphological changes of Hela cells were observed by the optical mi-croscope;the influence of SeO2 on the cell proliferation and vitality was examined by the MTT assay ;the flow cytometry was em-ployed to detect the cell apoptosis rate ;the expressions of caspase-3 and P53 proteins in Hela cells were determined by the Western blot analysis .Results Under the optical microscopy ,SeO2 generated the obvious influence on the cell growth morphology ,a large number of cells became rounded and shrunken ,and lost the normal form ,while the adherence cell number was evidently decreased and the proliferation was slowed down ;the MTT results showed that SeO2 markedly inhibited the cell proliferation and viability in a dose-dependent manner ,in which ,the cell apoptosis rates induced by the 0 ,1 .875 ,3 .750 ,7 .500 ,15 .000 and 30 .000 μmol/L con-centrations of SeO2 were 3 .12% ,30 .56% ,33 .42% ,37 .50% ,45 .43% and 69 .38% respectively ,which revealing the obviously in-creasing trend;the Western blot assay revealed that SeO2 could up-regulate the caspase-3 and P53 levels ,and reached the peak value at the concentration of 7 .500μmol/L .Conclusion SeO2 could induce the cervical cancer cell apoptosis possibly by up-regulating the expressions of caspase-3 and p53 in Hela cells .
