Effects of PEDF on phenotypic and immunologic function of dentritic cells
10.3969/j.issn.1671-8348.2014.12.024
- VernacularTitle:PEDF对小鼠树突细胞表型及免疫功能影响的实验研究
- Author:
Chanyu LI
;
Jian HAN
;
Lei HAN
;
Wei HUANG
;
Shuli ZHU
;
Xiuhui ZHENG
;
Jianxin GUO
;
Li LI
- Publication Type:Journal Article
- Keywords:
pigmentary epithelium derived factor;
dendritic cells;
phenotype;
immunity
- From:
Chongqing Medicine
2014;(12):1480-1482,1484
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of pigmentary epithelium derived factor (PDEF) on the phenotypic and immunologic function of murine-derived dentritic cells(BMDCs) .Methods Mononuclear cells(MNCs) isolated from murine bone marrow were cultured in RPMI1640 medium containing rmGM-CSF and rmIL-4 for 5 d ,and were divided into five groups .MNCs were stimulated for 3 d with either 50 ,100 ,200ng/mL PEDF ,1 μg/mL LPS(positive control) or RPMI1640(negative control) .The expression of CD11c ,CD80 and CD86 on DCs surface were analyzed by the fluorescence activated cell sorting (FCM ) .The ability of PEDF-induced BMDCs to stimulated T cell maturation were determined by the CCK-8 method and the level of IL-12 in the culture supernatant was detected by ELISA .Results The PEDF-treated BMDCs expressed high levels of CD11c ,CD80 and CD86 ,enhanced the immunolog-ical activities of T lymphocyte and its secretion of IL-12 when compared with untreated DCs .Conclusion PEDF can significantly up-regulate the expression of DCs immunological labelled molecule in in vitro cultured murine and increase its immunological com-petence .
