Expression profile of microRNAs in lung adenocarcinoma tissue and its expression in CD 133+ lung cancer cells
10.3969/j.issn.1671-8348.2014.12.001
- VernacularTitle:肺腺癌组织微RNA表达谱及在CD133＋肺癌细胞中的表达研究
- Author:
Xinchun HU
;
Jianjun XU
;
Dongliang YU
;
Jinhua PENG
;
Yiping WEI
- Publication Type:Journal Article
- Keywords:
lung neoplasms;
microRNAs;
CD133
- From:
Chongqing Medicine
2014;(12):1409-1411,1415
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression profile of microRNAs in the lung adenocarcinoma tissue and its expression in CD133+ lung cancer cells .Methods The specimens of adenocarcinoma tissue and pericarcinomatous tissue were collected for con-ducting the microRNA expression chip analysis .The flow cytometry(FCM ) was adopted to sort and culture CD133+ A549 cells in serum-free medium for 2 weeks .The positive rate of CD133+CD44+ in those cells was assayed .The expression of miRNA-892b and miRNA-4686 in CD133+ A549 cells were detected by real-time qPCR .Results Compared with the pericarcinomatous tissue ,27 miRNAs expressions in the lung adenocarcinoma tissue were up-regulated(P<0 .05) and 6 miRNAs expressions were down-regula-ted(P<0 .05) .The positive rate of cancer stem like cells CD 133+CD44+ was 75 .0% .The expression of miRNA-892b and miRNA-4686 in CD133+ A549 cells was higher than A549 cells(P<0 .01) .Conclusion Abnormal expression profile of miRNAs in lung ad-enocarcinoma tissue ,and miRNA-892b and miRNA-4686 may play a certain role to maintain the biological characteristics of CD133+ lung cancer cells .
