Effects of propofol on proliferation and apoptosis of HCC827 cells
10.7652/jdyxb201403017
- VernacularTitle:丙泊酚对HCC827肺癌细胞的增殖和凋亡的影响
- Author:
Jun CHEN
;
Wenhui ZHAO
;
Zhangjun SONG
;
Hongguang CHEN
;
Keliang XIE
;
Xixia ZHAO
;
Guangyan LEI
- Publication Type:Journal Article
- Keywords:
propofol;
lung cancer;
cell proliferation;
cell apoptosis
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2014;(3):361-363,384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of propofol on the proliferation and apoptosis of lung cancer cells as well as the related molecular mechanisms.Methods HCC827 cells were seeded in well plates with a density of 1×106 and then randomly divided into 5 groups:control group (group C),intralipid group (group E),low-dose propofol group (1.5μL/mL,group P1),medium-dose propofol group (2.2μL/mL,group P2),and high-dose propofol group (3.2μL/mL,group P3).At 6 h,24 h and 48 h after propofol treatment,the cells were collected to detect their proliferation and apoptosis.At 6h after treatment,the cells were collected for the measurement of Nrf2 mRNA and protein by RT-PCR and Western blot.Results Cell inhibition rate (IR)and apoptosis as well as Nrf2 mRNA and protein expressions in group E did not differ significantly from those in group C (P>0 .0 5 ).Compared with those in groups C and E,IR and apoptosis and Nrf2 mRNA and protein expressions were significantly increased in groups P1,P2 and P3 (P<0.05).Conclusion Propofol can inhibit the proliferation of cancer cells and promote cell apoptosis,thereby inhibiting the reoccurrence and metastasis of cancer cells probably via regulating the activation of Nrf2 expression.
