ESAT-6 enhances the expression of immune substances ofγδT cells through upregulating the expression of TLR-4
10.3969/j.issn.1671-8348.2014.13.001
- VernacularTitle:ESAT-6上调TLR-4通路增强人外周血γδT细胞产生淋巴因子的实验研究
- Author:
Guangming DAI
;
Hong WANG
;
Quan ZHEN
;
Xianzhi DU
- Publication Type:Journal Article
- Keywords:
ESAT-6;
TLR-4;
γδT cells;
cellular immunity
- From:
Chongqing Medicine
2014;(13):1537-1539,1542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of immune substances secreted by peripheral bloodγδT cells after stimulated by early secreted antigenic target-6(ESAT-6),and explore its mechanism of signaling pathway.Methods Peripheral blood mononucle-ar cells(PBMC)were collected from health human blood with the ficoll density gradient centrifugation method,and theγδT cells were separated from the PBMC with flow cytometry;the inhibitor of TLR-4 signaling pathway(E5564)was used to cocultured withγδT cells to inhibit the function of TLR-4,and the change of TLR-4 was analyzed by the methods of PCR and Western blot.ESAT-6 were used to stimulate theγδT cells,and the control group without any stimulating factor was established,then the expression levels of IL-17,TNF-α,IFN-γwere determined by ELISA method after 0、1、3、6、9 and 12 days.Results The results of PCR and Western blot showed that ESAT-6 could increase the expression of TLR-4(P<0.01);The results of ELISA showed that ESAT-6 could enhance the expression of IL-17,TNF-αand IFN-γ(P<0.01),and the inhibitor of TLR-4(E5564)could decrease the expres-sion of IL-17,INF-α,IFN-γ(P<0.01).Conclusion ESAT-6 can induceγδT cells to produce more IL-17,TNF-α,IFN-γ,and the mechanism of which maybe concerned with TLR-4 signaling pathway.
