Expression of cholecystokinin receptor B in a variety of human tumor tissues and cells
10.3969/j.issn.1671-8348.2014.13.019
- VernacularTitle:胆囊收缩素受体B在人体多种肿瘤组织和细胞中的表达
- Author:
Dongmei WANG
;
Chuang WANG
- Publication Type:Journal Article
- Keywords:
receptor,cholecystokinin B;
carcinoma;
gene expression
- From:
Chongqing Medicine
2014;(13):1584-1586,1590
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of cholecystokinin receptor B(CCK-BR)in a variety of human tumor tissues and cells.Methods RT-PCR and Western Blot were used to detect the expression of CCK-BR mRNA and protein in human gastric cancer AGS and SGC-7901cells,human hepatoma cancer HepG-2 cells,human neuroblastoma SH-SY5Y cells and human lung canc-er A549 and NCI-H446 cells,human prostate cancer DU145 cells,human bladder cancer T24 cells.CCK-BR expression was detected in the clinical cancer tissues including the stomach,breast,cervical,bladder,lung and liver and AGS and SGC-7901 cells by immuno-histochemical and immunocytochemical methods.Results CCK-BR mRNA and protein were express in the cytoplasm and cell mem-brane of the above 8 cell lines.Positive expression of CCK-BR was discovered in the above cancer tissues.Conclusion CCK-BR are expressed in a variety of human tumor tissues and cells.
