A study on preparation of recombinant human cystatin C and its polyclonal antibody
10.3969/j.issn.1673-4130.2014.10.001
- VernacularTitle:重组人胱抑素C及其多克隆抗体的制备
- Author:
Hongbin ZHANG
;
Jie WU
;
Yali WANG
;
Xia ZHOU
;
Jie WANG
;
Taicheng YANG
- Publication Type:Journal Article
- Keywords:
cystatin C;
antibodies;
preparation
- From:
International Journal of Laboratory Medicine
2014;(10):1233-1234,1237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the preparation of recombinant human cystatin C (Cys C ) and its polyclonal antibody . Methods The gene sequences encoding Cys C were designed according to characteristics of E .coli protein-coding ,and the target gene was artificially synthesized and expressed in E .coli .The target protein was purified by affinity chromatography and was used to immunize the New Zealand white rabbits .Western blot and enzyme-linked immunosorbent assay(ELISA) were adopted to detect the recombinant human Cys C and its polyclonal antibodies in the rabbits serum .Results Purity of prepared recombinant human Cys C was more than 90% .Anti-recombinant human Cys C antibody showed good binding activities and specificity .Conclusion Prepared recombinant human Cys C and its antibody can be used in further experimental research and clinical application .
