Radiosensitization effects of celecoxib on glioblastoma: comparison with oligodendrocyte cell line

VernacularTitle:塞来昔布对正常脑胶质细胞和脑胶质瘤细胞放射增敏效应的比较及其机制
Author: Liangqin NIE ; Juying ZHOU ; Lili WANG ; Xiaoting XU ; Songbing QIN
Publication Type:Journal Article
Keywords: Cox-2 inhibitor; Celecoxib; Radiosensitivity; Glioblastoma
From: Chinese Journal of Radiological Medicine and Protection 2014;34(5):342-344,375
CountryChina
Language:Chinese
Abstract: Objective To compare the radiosensitivity effect of celecoxib on oln93 and u373 cells,and to explore the related mechanism.Methods Both oln93 cells and u373 cells were respectively divided into control group,drug group,radiation group and combined group when treated with celecoxib and irradiation.Cell survival ratio was evaluated by MTT assay and clogenic assay.Flow cytometry and Western blot assay were used to measure cell cycle and protein expression.Results Celecoxib had a similar effect on oln93 and u373 cells in enhancing the radiosensitivity (t =2.215-30.996,P ＜ 0.05 ; t =0.383-11.732,P＜0.05)and blocking cellcycle in G0/G1(t=-6.1-5.141,P＜0.05).Compared with the radiation group,the combined group showed S phase arrest(t =-18.174,P ＜ 0.05),and increase of Cyclin A protein (t =-8.087,P ＜ 0.05) in oln93 cells,and G2/M arrest and decrease of Cyclin B1 and DNA-PKcs and MRE11 protein (t =-8.838-10.45,P ＜ 0.05) in u373 cells.Conclusions Celecoxib illustrates a more sensitive radiosensitivity to u373 cells by regulating its cell cycle and DNA damage repair.