Preparation and evaluation of quality control serum of autoantibodies threshold
10.3969/j.issn.1673-4130.2014.10.041
- VernacularTitle:自身抗体临界值质控血清的制备及评价
- Author:
Liangqiong CHEN
;
Feiyue JI
;
Yixiang CUI
- Publication Type:Journal Article
- Keywords:
autoantibodies;
quality control;
threshold
- From:
International Journal of Laboratory Medicine
2014;(10):1326-1326,1328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and evaluate the preparation of quality control serum of autoantibodies threshold . Methods Donors′ serum blood were placed in 56 ℃ for 30 min to inactivate ,followed by centrifuged to remove the precipitates ,and then multi-batch quality control material in detection kit were detected in dilution ratios of 1∶2 ,1∶4 ,1∶6 .Results Anti-RNP/Sm antibody(+ + + ):after 1∶2 dilution ,the coloration degree was + + + ;after 1∶4 ,1∶6 dilution ,the coloration degree was ++ .Anti-SS-A antibody(+ + to + + + ):after 1∶2 dilution ,the coloration degree was + + ;after 1∶4 ,1∶6 dilution ,the colora-tion degree was + to + + .Anti-RO-52 antibody(+ + to + + + ):after 1∶2 dilution ,the coloration degree was + to + + ;after 1∶4 ,1∶6 dilution ,the coloration degree was + .Anti-nucleosome antibodies :positive were found in dilution of 1∶1 ,1∶2;after 1∶4 ,1∶6 dilution ,the coloration was negative .Conclusion Preparation of quality control serum of autoantibodies is convenient , reliable ,and can meet the needs of clinical detection of internal quality control .
