Extraction of Volatile Oil from Camphor Tree and Study on Prepration and Identification of ;Its β-cycoldextrin Inclusion Compound
10.3969/j.issn.1005-5304.2014.06.027
- VernacularTitle:樟树挥发油的提取及包合物制备与鉴定研究
- Author:
Ying PENG
;
Ting OUYANG
;
Hong YAN
;
Maijiao PENG
;
Xinhua XIA
;
Jing YANG
;
Genhua XU
- Publication Type:Journal Article
- Keywords:
camphor tree;
volatile oil;
β-cyclodextrin inclusion
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(6):86-88,94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the content of volatile oil from old leaves, tender leaves, fallen leaves and seeds of cinnamomum camphora tree in Hunan, China. To prepare and identify the volatile oil of its β-cycoldextrin inclusion compound. Methods The volatile oil was extracted by water-steam distillation. With inclusion rate as the index, trituration method, saturated water solution method and ultrasound method were compared. The optimum conditions were investigated by the orthogonal test. The inclusion compound was identified by thin-layer chromatography (TLC), ultraviolet visible spectrum (UV-Vis) and differential scanning calorimetry (DSC). Results From June to July, the average content of volatile oil extracted from old leaves, tender leaves, fallen leaves and seeds were 1.58%, 1.52%, 0.84% and 1.39%, respectively. The optimum preparation conditions were as follows:the ratio of β-cyclodextrin and volatile oil was 10∶1, the adding water was 4 times and inclusing time was 2 h. Before and after inclusion, the spectrum of TLC and UV-Vis of volatile oil showed no obvious change. The DSC of inclusion compound,β-cycoldextrin, volatile oil and the mixture had significant differences. Conclusion The amount of the volatile oil from old leaves is higher. The optimized condition of inclusion is stable and reasonable.
