Establishment and application of determination of glyphosate poisoning method by UV spectrophotometry
10.3969/j.issn.1673-4130.2014.08.038
- VernacularTitle:紫外分光光度法检测血清草甘膦中毒方法的建立和应用
- Author:
Shubin WU
;
Guohong LIU
;
Xinru WANG
;
Fadong ZHANG
;
Ziru CHEN
;
Shuming DU
- Publication Type:Journal Article
- Keywords:
serum;
glyphosate;
poisoning;
nitrosylation;
ultraviolet spectrophotometry
- From:
International Journal of Laboratory Medicine
2014;(8):1024-1025,1028
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a qualitative and quantitative determination of glyphosate in serum using ultraviolet spectro-photometry(UV) to provide basis for clinical diagnosing and treating glyphosate poisoning .Methods The mixture of 0 .5 mL serum and 0 .2 mL 10% methanol solution of perchloric acid was shocked and centrifuged with 10 000 r/min for 5 min .A nitrosyla-tion reaction conducted on supernatant and 50 μL serum nitrosylation liquid was detected by UV scanning .Results The results of serum theophylline absorption maxima was(243 ± l) nm and the concentration of 10 .0-60 .0 μg/mL range linear regression equa-tion was Y=0 .0173 8X+0 .036 3(r= 0 .999 8) .The recovery rate was from 85 .5% to 102 .4% and the relative standard deviation (RSD) was from 3 .50% to 4 .90% .The intra-day and inter-day RSD were 3 .79% -5 .10% and 3 .88% -4 .55% .The minimum de-tectable concentration was 5μg/mL .Conclusion This method is simple ,rapid and accurate results for detecting glyphosate poison-ing .
