Study on error analysis during detecting aflatoxin B1 in food by using electrochemical immunosensor
10.3969/j.issn.1673-4130.2014.08.002
- VernacularTitle:电化学免疫传感器检测样品中黄曲霉毒素B1的误差分析
- Author:
Tian FENG
;
Xian ZHANG
;
Xianxian YANG
;
Chaorui LI
- Publication Type:Journal Article
- Keywords:
electrochemical immunosensor;
aflatoxin B1;
error analysis
- From:
International Journal of Laboratory Medicine
2014;(8):947-948,951
- CountryChina
- Language:Chinese
-
Abstract:
Objective To expand the application of electrochemical immunosensor during deleting aflatoxin B1 in foods and feeds through analyzing impacts of the time of antibody incubation and sample preparation .Methods T he double self-assembly immu-nosensor combined with aflatoxin B1 and carboxylated single-walled carbon nanotubes (SWNTs) was characterized by cyclic volta-mmetry and impacts of the time of antibody incubation and sample preparation methods were investigated .Results The signal in-creased gradually following the increasing time of antibody incubation and reached a plateau at 90 min and sample preparation meth-ods showed a comparatively large impact on results .Additionally ,the crude extractions purified through removing interfering com-pounds by immunoaffinity column could effectively eliminate the interference effects of sample matrix .Conclusion Deleting aflatox-in B1 by electrochemical immunosensor is characterized by various features ,such as fast ,simple and low detection limits .The pres-ent study shows that stability of the electrochemical immunosensor is affected by the time of antibody incubation and sample prepa-ration .
