Amniotic cell culture and karyotype analysis of 1 016 pregnant women in second trimester
10.3969/j.issn.1673-4130.2014.09.021
- VernacularTitle:1016例孕中期孕妇羊水细胞的培养及染色体核型分析
- Author:
Yongjian LI
;
Liqiong YAN
;
Yijian PANG
- Publication Type:Journal Article
- Keywords:
prenatal diagnosis;
amniocentesis;
amniotic fluid;
cell culture techniques;
karyotype
- From:
International Journal of Laboratory Medicine
2014;(9):1138-1139,1142
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application value of amniotic cell culture and karyotype analysis in prenatal diagnosis . Methods 1 016 pregnant women in second trimester were subject to amniocentesis under the guidance of B-type ultrasonic inspec-tion ,and the cell culture and karyotype analysis were performed on the amniotic fluid which had been drawn out .Results Among 1 016 pregnant women ,1 011(99 .5% ) succeeded in the first operation of amniocentesis and cell culture .The detection rate of fetal chromosomal abnormal karyotype was 10 .3% (105/1 016) ,in which 8 .3% (85/1 016) of structural abnormality and 2 .0%(20/1 016) of quantity abnormality .856 cases were received follow-up .Conclusion Amniotic fluid cell culture and karyotype analy-sis is a safe and reliable method for prenatal diagnosis .
