Content Comparison of Polysaccharide and Protein in Male and Female Trichosanthis Radix
- VernacularTitle:基于植株性别差异的天花粉多糖、蛋白质含量比较
- Author:
Bo ZHANG
;
Hongyan LIU
;
Jia LI
;
Yanli PENG
- Publication Type:Journal Article
- Keywords:
Trichosanthis Radix;
Male and female plant;
Polysaccharide;
Tichosanthin( TCS)
- From:
China Pharmacist
2014;(4):617-618,619
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the contents of polysaccharide and protein in male and female Trichosanthis Radix to provide reasonable explanation for that male plant was better. Methods:Polysaccharides in Trichosanthis Radix were obtained by water-extrac-tion and ethanol-precipitation method, and the content was determined by anthrone-sulfuric acid method. Tichosanthin ( TCS) was ob-tained by hydrochloric acid extraction and acetone precipitation. Based on the light absorption of Coomassie brilliant blue G-250 com-bined with proteins, the content of TCS was detected. Results: The polysaccharides content in male and female Trichosanthis Radix was 2. 628% and 3. 237%, TCS content was 0. 193% and 0. 280%, respectively. Conclusion: The contents of polysaccharides and TCS in male plant are higher than those in female plant. The male plant is a better source for Trichosanthis Radix.
