Protective effect of schizandrin B against oxidative damage of UVB irradiated HaCaT cells and its molecular mechanism

VernacularTitle:五味子乙素对UVB辐射诱导HaCat细胞凋亡的抑制作用及其机制
Author: Bo CAO ; Cong NIU ; Tao LU ; Jie HU ; Hong CHEN
Publication Type:Journal Article
Keywords: natural products; schizandrin B ( SchB); HaCaT cells; UVB radiation; apoptosis; mechanism
From: Chinese Pharmacological Bulletin 2014;(4):523-526,527
CountryChina
Language:Chinese
Abstract: Aim To investigate the inhibitory effect of schizandrin B( SchB) on ultraviolet radiation b ( UVB) radiation-induced apoptosis of HaCaT cells. Methods Methyl thiazolyl tetrazolium ( MTT ) assay was used to examine the effect of SchB on cell viability recovery. Cell apoptosis and necrosis were measured by Ho-chest33342 staining. The p53, p21 and Caspase-3 mRNA expressions were examined by RT-PCR. Results In this study, we found that Sch B attenuated UVB-in-duced toxicity in HaCaT cells. Through Hoechst 33342 stain, we visualized that SchB could inhibit UVB-in-duced HaCaT cell death. The result demonstrated that p53 , p21 and Caspase-3 mRNA levels decreased com-pared with the control group. Conclusions Sch B at-tenuates the UVB-induced toxicity of HaCaT by inhibi-ting apoptotic gene expression. It plays a role in anti-photoaging.