Correlation between Toll-like receptor 4, C-Jun N-terminal kinase,tumor necrosis factor-α,Leptin changes and chronic intermittent hypoxia of rats
- VernacularTitle:慢性间歇性缺氧大鼠脂肪组织中TLR4、JNK和血中TNF-α、Leptin的变化
- Author:
Guanhan LONG
;
Meixi CHEN
;
Jun CHENG
- Publication Type:Journal Article
- Keywords:
chronic intermittent hypoxia;
Toll-like receptor 4;
C-Jun N-terminal kinase;
tumor necrosis factor-α;
Leptin
- From:
Acta Universitatis Medicinalis Anhui
2014;(2):168-171,172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between TLR4,C-Jun N-terminal kinase,tumor necrosis factor-α(TNF-α),Leptin changes and chronic intermittent hypoxia(CIH). Methods Twenty-four SD rats were divided into 3 groups (n=8):control group,CIH 6 weeks group, CIH 8 weeks group. After the modeling,the change of blood pressure and renal artery were observed by the use of non-invasive blood pressure analyzer and HE staining. ELISA was employed to determine the production of TNF-αand Leptin in sera of these animals. The expressions of TLR4 and JNK mRNA were detected by real time quantitativer everse transcription-polymerase chain reaction ( QRT-PCR) method in the adipose tissue. Results The blood pressure in intermittent hypoxia group was signifi-cantly higher than those in control group(P<0.01), and the renal artery pathological changes in intermittent hy-poxia group were significantly heavier than those in control group;the content of TNF-α and Leptin in intermittent hypoxia was significantly higher than those in control group(P<0.01),and the content of TNF-α and Leptin was significantly increased with the days of hypoxia;the expression of TLR4 and JNK at mRNA levels in intermittent hy-poxia was significantly higher than those in control group(P<0.01),and the expression of TLR4 and JNK at mR-NA levels was significantly increased with the days of hypoxia. Conclusion TLR4,JNK,TNF-α,Leptin may be in-volved in the inflammatory response of chronic intermittent hypoxia in rats.
