Protective effects of hydrogen-rich saline on systemic oxidative stress in rats with severe traumatic brain injury
10.3969/j.issn.1671-8348.2014.08.017
- VernacularTitle:富氢生理盐水对大鼠重度颅脑损伤后氧化应激保护作用的研究
- Author:
Qiling XU
;
Li YAN
- Publication Type:Journal Article
- Keywords:
craniocerebral injury;
rats,Wistar;
oxidative,stress;
reactive oxygen species
- From:
Chongqing Medicine
2014;(8):943-945,948
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of hydrogen-rich saline on systemic oxidative stress in rats with severe traumatic brain injury(STBI) .Methods Male Wistar rats(n=60) were divided into three groups randomly :sham group ,STBI+normal saline group ,STBI+ hydrogen-rich saline group .STBI model was induced by controlled cortical impact injury .Hydrogen-rich saline were intraperitoneally administered at 5 min after STBI operation .Plasma malondialdehyde (MDA ) ,Superoxide Dis-mutase(SOD) activity and glutathione peroxidase(GSH-PX) activity were measured before TBI operation ,12 ,24 h and 48 h after TBI operation .Results The level of MDA in STBI group plasma was significantly elevated from 24 h to 48 h after STBI operation (P< 0 .05) .Hydrogen-rich saline treatment significantly attenuated the increase of MDA level in STBI operation animals (P<0 .05) .The activities of SOD and GSH-PX were slightly elevated at 6 h after TBI operation ,but decreased significantly from 24 h to 48 h after STBI operation(P<0 .05) .Hydrogen-rich saline treatment significantly increased the activities of SOD and GSH-PX after STBI operation(P<0 .05) .Conclusion Hydrogen-rich saline could exert a protective effect against STBI via reducing oxidative stress .Molecular hydrogen might be a more effective therapeutic strategy for TBI patients .
