Synergetic Inhibition Effects of Methioninase and Cisplatin against the Growth of Human Lung Adenocarcinoma Cell Line GLC
- VernacularTitle:蛋氨酸酶联合顺铂协同抑制人肺腺癌细胞GLC生长作用
- Author:
Yuexin HU
;
Tianyong XU
;
Yuwen ZHANG
;
Yu ZHAO
;
Changfu TIAN
- Publication Type:Journal Article
- Keywords:
Inhibition;
Methionine lyase;
Synergy;
Cisplatin;
Lung adenocarcinoma cell
- From:
Journal of Kunming Medical University
2014;(1):5-7,20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To purify recombinant methioninase and investigate the synergetic inhibitory effect of methioninase and cisplatin on the proliferation of human lung adenocarcinoma cell line GLC. Methods Recombinant methioninase was purified with GST-column from supernatant after ultrasonic disruption of cultured Escherichia coli in the prokaryotic expression system pGEX-4T-1-Met/Dh5a. MTT assay was used to determine the inhibition rate of methioninase in combined with cisplatin on cell proliferation,and their synergistic effect was evaluated by using the q value judge method. Results The concentration of recombinant methionine cleaving enzyme was 0.22 mg/mL, the purity was 95%, and the activity was 0.568 IU/mg. After 72 h culturing, the inhibition rate of cisplatin methionine was 24.80%and 27.49%respectively,while the inhibition rate of the combined drugs was 66.80% ( =1.47>1.15) which showed a significant synergistic effect. Conclusion Both methioninase and cisplatin have the inhibition effect,and the combined drugs display a significant synergistic effect on the proliferation of GLC.
