Enhancement of cetuximab on radiosensitivity of colorectal cancer cells exposed to 125I seeds

VernacularTitle:西妥昔单抗增加结直肠癌细胞对125I粒子持续低剂量率照射的敏感性
Author: Jingjia LIU ; Hao WANG ; Ang QU ; Jinna LI ; Yong ZHAO ; Junjie WANG
Publication Type:Journal Article
Keywords: C225; Continuous low dose rate radiation; Radiosensitization; Colorectal cancer cell; Cell apoptosis
From: Chinese Journal of Radiological Medicine and Protection 2014;34(1):26-29,33
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of cetuximab (C225) on the radiosensitivity of colorectal cancer cells CL187 and underlying mechanism.Methods Cell survival was detected by colony forming assay.The levels of apoptosis and cell cycle distribution were determined by flow cytometer.The mitotic ratio was measured by Wright' s-Giemsa mixed coloring method.The protein levels of Bax and Bcl2 were detected by Western blot.Results The sensitizing enhancement ratio of C225 was approximately 1.4.C225 treatment and 125I seed radiation induced G1 cell cycle arrest individually.C225 increased the radiation-induced apoptosis (t =6.6,P ＜ 0.05) and cellular Bax/Bcl-2 ratio (t =9.4,P ＜ 0.05),but did not increase radiation-induced G1 arrest.In addition,there was no difference in mitotic index among different groups.Conclusions C225 sensitizes CL187 to 125I seed irradiation,which might be related with increase of radiation-induced apoptosis.