Determination of Gallic Acid in Yuanhu Zhitong Soft Capsule by HPLC
10.3969/j.issn.1005-5304.2014.04.025
- VernacularTitle:HPLC测定元胡止痛软胶囊中没食子酸含量
- Author:
Maobo DU
;
Shuzhi LIU
- Publication Type:Journal Article
- Keywords:
Yuanhu Zhitong Soft Capsule;
gallic acid;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(4):80-81,85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the determination method of gallic acid in Yuanhu Zhitong Soft Capsule by HPLC. Methods Chromatography was performed on a Unitary C18 column (250 mm× 4.6 mm, 5 μm) at 254 nm. The mobile phase consisted of a mixture of methanol and 0.5%phosphate acid (5∶95) at flow rate of 1.0 mL/min. Results The standard curve of linear relationship was 0.024 8-0.496 μg and r=0.999 9. The RSD of precision, repeatability and stability was 0.27%, 0.21%, 1.40%, respectively. The average recovery was 99.81%, RSD was 0.72%. Conclusion The established method was accurate and reliable for the determination of gallic acid in Yuanhu Zhitong Soft Capsule.
