Effect of Yiqi Jianpi Herb on Content of NPY, VIP and Expression of Mapk14 mRNA of Rats with Spleen-qi Deficiency
10.3969/j.issn.1005-5304.2014.04.019
- VernacularTitle:益气健脾中药对脾气虚大鼠神经肽Y、血管活性肠肽和丝裂原活化蛋白激酶14基因表达的影响
- Author:
Yongqiang DUAN
;
Weidong CHENG
;
Juan DU
;
Xiaoyi YANG
;
Liang LIU
;
Zihan GONG
;
Liming ZHU
;
Yingxia CHENG
;
Lanzhen LI
- Publication Type:Journal Article
- Keywords:
spleen-qi deficiency;
NPY;
vasoactive intestinal peptide;
Mapk;
Sijunzi decoction;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(4):59-62,66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Yiqi Jianpi herb on content of NPY, VIP and expression of Mapk14 mRNA of rats with spleen-qi deficiency. Methods Rats were randomly divided into normal groups, model group (observed on 7 d, 14 d and 21 d), treatment group of Yiqi Jianpi herb, 10 rats in each group. The spleen-qi deficient model rats were made by rhubarb, exhaustive and hungry method, and treatment group was treated with Sijunzi decoction (20 g/kg) for 21 d, then the content of NPY, VIP in serum and small intestine were evaluated with radioimmunoassay, and expression of Mapk14 mRNA in small intestine tissue was evaluated with real time fluorescent quantitative PCR. Results In model group, content of NPY was much lower than that of normal group (P<0.05), content of VIP and relative expression of Mapk14 mRNA in small intestine tissue were much higher than that of normal group (P<0.05), the difference was obvious in 21 d group. Compared with model 21 d group, content of NPY was increased (P<0.05), content of VIP and relative expression of Mapk14 mRNA in small intestine tissue were decreased (P<0.05) in treatment group. Conclusion Yiqi Jianpi herb has functions of adjusting NPY, VIP secretion and inhibiting abnormal expression of Mapk14 mRNA.
