Determination of Paeoniflorin in Fufang Chishao Granula by HPLC
10.3969/j.issn.1005-5304.2014.04.024
- VernacularTitle:HPLC测定复方赤芍颗粒中芍药苷含量
- Author:
Yinyang BI
;
Jiwen LI
;
Xiaoshuan LIU
;
Xixiang LI
;
Haining LIANG
- Publication Type:Journal Article
- Keywords:
Fufang Chishao Granula;
paeoniflorin;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(4):78-79,80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for determination of paeoniflorin in Fufang Chishao Granula. Methods The separation was performed on a Waters C18 column (250 mm× 4.6 mm, 5μm) with acetonitrile-water (30∶70) as the mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was set at 230 nm. Results Paeoniflorin showed good linearity (r=0.999 9) in the range of 0.266-5.32 μg, and the average recovery was 98.00%, RSD=2.22%. Conclusion The method is convenient, sensitive, accurate and repeatable. It can be applied to determination of paeoniflorin in Fufang Chishao Granula and control the quality of the preparation.
