Effect of Chinese Yam Polysaccharide on Expression of HIF-1α and VEGF in Rats with Renal Ischemia Reperfusion Injury
10.3969/j.issn.1005-5304.2014.02.016
- VernacularTitle:山药多糖对肾缺血再灌注损伤大鼠肾组织缺氧诱导因子-1α和血管内皮生长因子表达的影响
- Author:
Qun TANG
;
Hua WU
;
Jiushi LEI
;
Xi ZHANG
;
Lanlan BU
- Publication Type:Journal Article
- Keywords:
Chinese yam polysaccharide;
ischemia reperfusion injury;
hypoxia inducible factor-1α;
vascular endothelial growth factor;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(2):56-58,62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Chinese yam polysaccharide on the expression of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in rats with renal ischemia reperfusion injury. Methods SD rats were randomly divided into sham operation group, model group and Chinese yam polysaccharide group. Renal ischemia reperfusion injury model in rats was prepared. Seven days before operation, Chinese yam polysaccharide group was gastrically administrated with yam polysaccharide (200 mg/kg) daily, sham operation group and model group with same volume of saline respectively. BUN and SCr were detected postoperative 6 h, the protein expression of HIF-1αin renal tissue was detected by Western Blot, and HIF-1αand VEGF mRNA expression in renal tissue were detected by RT-PCR. Results Compared with sham operation group, serum levels of BUN and SCr of model group and Chinese yam polysaccharides group increased (P<0.01), and the mRNA and protein expression of HIF-1α, mRNA expression of VEGF in renal tissue increased (P<0.01). Compared with model group, serum levels of BUN and SCr of Chinese yam polysaccharide group decreased (P<0.01), and HIF-1α mRNA and protein expression, VEGF mRNA expression in renal tissue increased (P<0.01). Conclusion Chinese yam polysaccharide has obvious protective effect on renal ischemia reperfusion injury, which may be related with up-regulation of HIF-1αexpression and induction of VEGF expression.
