Determination of Pinitol in Fufang Kushen Injection by HPLC-ELSD
10.3969/j.issn.1005-5304.2014.02.024
- VernacularTitle:HPLC-ELSD测定复方苦参注射液中松醇含量
- Author:
Bowen LI
;
Siju ZHANG
;
Hongying ZHI
;
Lina HAI
;
Wenjie QIN
;
Anping LI
- Publication Type:Journal Article
- Keywords:
Fufang Kushen Injection;
HPLC-ELSD;
pinitol
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(2):83-84,85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC-ELSD method for the determination of pinitol in Fufang Kushen Injection. Methods Chromatographic condition was as follows:Cosmosil Sugar-D column (250 mm×4.6 mm, 5 μm), the mobile phase consisting of acetonitrile-water in gradient mode, the temperature of the drift tube of 77 ℃, and the gas flow rate of 2.0 L/min. Results The standard curve of pinitol was rectilinear within the range of 0.5-10.0 μg, r=0.999 6 (n=5). The average recovery was 97.30%(RSD=1.29%, n=5). Conclusion The method is simple and rapid, the result is accurate, reliable and reproducible.
