Study on Quality Standard of Qingshen Granules
10.3969/j.issn.1005-5304.2014.03.022
- VernacularTitle:清肾颗粒质量标准研究
- Author:
Jinmin SHI
;
Jiarong GAO
;
Raorao LI
;
Lu JIA
;
Xuezhu GU
- Publication Type:Journal Article
- Keywords:
Qingshen Granules;
quality standard;
TLC;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(3):75-77,78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality standard of Qingshen Granules. Methods Thin-layer chrmatography was used to identify Rheum officinale Baill., Hedyotis Diffusa Wild. and Coptis chinensis Franch.. The contents of rhein, emodin and chrysophanol were determined by HPLC. The HPLC consisted of Kromasil C18 (4.6 mm×250 mm, 5 μm), methanol-0.1% phosphonic acid (70∶30) as mobile phase, flow rate of 1.0 mL/min, detection wavelength at 245 nm, and the column temperature was 30 ℃. Results The results of TLC showed that relevant spots were clear without interference against the negative sample. The calibration curves for rhein, emodin and chrysophanol were found to be linear within the range of 0.003 3-0.42 μg (r =0.999 9), 0.008 0-0.51 μg (r=1.000 0), 0.009 9-0.32 μg (r=1.000 0), respectively. The average recoveries were 98.84%, 99.04% and 100.35% with RSD of 1.30% (n=6), 1.34% (n=6) and 1.89% (n=6), respectively. Conclusion The methods are accurate and quick in qualitative identification and quantitative assay, and can be used for the quality control of Qingshen Granules.
