Expression and clinicopathologic significance of Golgi phosphoprotein 3 in ovarian serous cystadenocarcinoma
10.3760/cma.j.issn.1006-9801.2013.12.008
- VernacularTitle:卵巢浆液性囊腺癌组织中高尔基磷酸化蛋白3的表达及其意义
- Author:
Fengli LI
;
Yingchun MA
;
Haiying CHEN
;
Aixia WANG
;
Weiguo JIN
;
Shuangfeng CHEN
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Cystadenocarcinoma,serous;
Golgi phosphoprotein 3;
Polymerase chain reaction;
Blotting,western
- From:
Cancer Research and Clinic
2013;25(12):816-818,822
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Golgi phosphoprotein 3 (GOLPH3) at protein and mRNA levels in serous epitheial ovarian carcinoma and its significance.Methods The expression of GOLPH3 at protein and mRNA levels were evaluaed by Western blot and Real time RT-PCR,respectively,in 42 cases of serous epithelium ovarian carcinoma,14 cases of benign serous ovarian tumors and 7 cases of normal ovarian epithelium tissues.Results GOLPH3 mRNA was significantly higher from 2.97 to 7.04 fold in ovarian serous cystadenocarcinoma tissues compared with ovarian serous carcinoma tissues (P ＜ 0.05).There was no positive expression of GOLPH3 protein in normal ovarian epithelium tissues.GOLPH3 protein positive expression rate was higher in ovarian serous cystadenocarcinoma (73.81 ％) than that in ovarian serous carcinoma (35.71％) (P ＜ 0.01).GOLPH3 protein was correlated with the pathologic differentiation (P =0.019),the FIGO stage (P =0.042),and lymphatic metastasis (P =0.000),but was not associated with age (P =0,881).The positive rate and overexpression of GOLPH3 in poor differentiated grade group were higher than that in well and middle differentiated grade group (P ＜ 0.05).Conclusions GOLPH3 may play an important role on the development of ovarian serous cystadenocarcinoma,and may be a potential target of gene therapy.
