Studies on distribution and imaging of new 99Tcm-labelled RGD peptide in synovial pannus of mice
10.3760/cma.j.issn.1007-7480.2013.10.004
- VernacularTitle:采用99Tcm标记的新精氨酸-甘氨酸-天冬氨酸多肽序列显示胶原诱导性关节炎大鼠滑膜血管翳形成的研究
- Author:
Gula DA
;
Hongbin LI
;
Yong WANG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Arthritis,experimental;
Vascular endothelial growth factors;
Bevacizumab;
Arginine-Glycin-Aspartic aicd
- From:
Chinese Journal of Rheumatology
2013;17(10):661-666,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the distribution and imaging of radiolabelled arginine-glycineaspartic(RGD)peptide(99Tcm-3P4-RGD2)in collagen-induced arthritis(CIA),and to investigate the possibility of new markers for monitoring the angiogenesis of rheumatoid arthritis(RA)before and after treatment by single photon emission computed tomography(SPECT)labelled with 99Tcm-3P4-RGD2.Methods Type Ⅱ chicken collagen was injected into the tails of Wistar rats to establish arthritis models.The CIA-Wistar rats were randomly divided into three group:the Avastin group,the recombinant human tumor necrosis factor-α receptor Ⅱ group,and the blank control group.The changes of the arthritis index,joint pad thickness and radiological of T/NT before and after treatment were observed.The level of vascular endothelial growth factor(VEGF)in the serum was measured by enzyme-linked immuno sorbent assay(ELISA).The back metapedes pathological section was analyzed by Hematoxylin and Eosin stain(HE)and Safranin O stain as well as arthritic pathology index was also scored.The synovial expression level of VEGF was measured by immuno-histochemistry.The stain level of VEGF was assayed by semiquantitative approach.T test was applied for the comparison of two intergroup samples.Results Abnormal radioactive concentration appeared in the arthritis of the CIA-Wistar rats(1.40±0.17,1.32±0.20,1.30±0.08,P＜0.05).Through the treatment of Avastin and recombinant human tumor necrosis factor-α receptor Ⅱ,the radiological of T/NT decreased significantly(0.43±0.14,0.40±0.12,t=17.710,16.812,P＜0.05).After the treatment with Avastin and recombinant human tumor necrosis factor-α receptor Ⅱ,the severity of synovial pannus,joint pad thickness,arthritis index and the expression of VEGF were all decreased.The radiological of T/NT was positively correlated with the indices mentioned above(r=0.753,0.800,0.892,all P＜0.01 respectively with the the rhTNFR:Fc group)(r=0.701,P=0.001;r=0.502,P=0.024;r=0.481,P=0.032 individually with the rhTNFR:Fc group).Conclusion When 99Tcm-3P4-RGD2 is injected through the tail vein of Wistar rats,it can show the pathological changes of arthritis clearly.99Tcm-3P4-RGD2may be the new eikonogen to monitor the angiogenesis of RA before and after treatment.
