Selection and functional identification of siRNA targeting of HOXA7
10.3760/cma.j.issn.1006-9801.2013.09.002
- VernacularTitle:靶向同源盒基因A7的小干扰RNA筛选及功能鉴定
- Author:
Baohui YIN
;
Xiuhong JIA
;
Jianchang LI
;
Youjie LI
- Publication Type:Journal Article
- Keywords:
Genes,homeobox;
RNA interference;
Lung cancer LETP-a-2 cells
- From:
Cancer Research and Clinic
2013;25(9):581-584,587
- CountryChina
- Language:Chinese
-
Abstract:
Objective To design and screen small interefere RNA (siRNA) targeting of HOXA7,and to investigate the effect of the siRNA on human lung cancer LETP-a-2 cells proliferation and apoptosis in vitro.Methods Three pairs of siRNA targeting of HOXA7 and one pair of siRNA for negative control were transfected respectively into LETP-a-2 cells through cationic liposome.The mRNA and proteion expression levels of HOXA7 were observed by reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis.The effect of HOXA7 siRNA on growth and apoptosis of LETP-a-2 cells were measured by MTT and flow cytometry.Results All the three pairs of siRNA could inhibit HOXA7 expression effectively,among which siRNA2 got the best effects,the silence rates were (57.344±4.743) ％ on mRNA level and (52.219±0.550) ％ on protein leval.The proliferation was inhibited and the apoptosis was promoted by the siRNA targeting HOXA7 in LETP-a-2 cells,among which siRNA2 got the favourite results,the inhibitory rate was (48.144±4.992) ％ and the apoptosis rate was (26.613±0.612) ％.Conclusion The siRNA2 targeting of HOXA7 enrolls in promoting apoptosis and inhibiting grows of LETP-a-2 cells,indicating that manipulation of HOXA7 expression may be a potential therapeutic strategy for cancer.
