Effect of Simiaoyongan Decoction with Shengmai Decoction on Apoptosis of Hypoxia/Reoxygenation Neonatal Rat Cardiomyocytes
10.3969/j.issn.1005-5304.2013.12.013
- VernacularTitle:四妙勇安汤合生脉饮对缺氧/复氧乳鼠心肌细胞凋亡的影响
- Author:
Huiqing YU
;
Yunhui MENG
;
Yongxin LI
;
Qiang WANG
;
Zhen LIU
;
Xiaoyan LIU
;
Wuwei LI
- Publication Type:Journal Article
- Keywords:
Simiaoyongan decoction;
Shengmai decoction;
apoptosis of myocardial cells;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(12):35-36,37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Simiaoyongan decoction with Shengmai decoction on apoptosis of hypoxia/reoxygenation model of primary cultured neonatal rat cardiomyocytes. Methods The combination of trypsin and collagenase was used to isolate neonatal rat myocardial cells. Hypoxia/reoxygenation model of cardiomyocytes was made by high purity nitrogen gas. Primary cultured neonatal rat cardiomyocytes were randomly divided into control group, model group and treatment group (treated with Simiaoyongan decoction and Shengmai decoction). The myocardial apoptotic rates were detected by Annexin V-FITC/PI double-staining method. Results Compared with the control group, the apoptotic rate of model group and treatment group was high (P<0.05). Drug intervention lowered the apoptotic rate compared with the model group, the difference was significant (P <0.05). Conclusion The alcohol sedimentation solution of Simiaoyongan decoction with Shengmai decoction played a protective role on hypoxia/reoxygenation cardiomyocytes.
