HPLC Determination of Aloin and Aloeemodin in Barbodos Aloe
10.3969/j.issn.1005-5304.2013.12.022
- VernacularTitle:HPLC测定药用芦荟中芦荟苷和芦荟大黄素含量
- Author:
Guilong PENG
;
Guangming ZHOU
;
Hongying QIN
- Publication Type:Journal Article
- Keywords:
ionic liquid;
ultrasonic-assisted;
HPLC;
Barbodos Aloe;
Aloin;
Aloeemodin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(12):59-60,61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and determine Aloin and Aloeemodin in Barbodos Aloe by ionic liquid-based ultrasonic-assisted extraction coupled with high performance liquid chromatography, with 1-butyl-3-methylimidazolium chloride ([BMIM]Br) solution as the extraction solvent. Methods The separation was performed on Phenomenex C18 column (250 mm×4.6 mm, 5 μm) with detection wavelength of 360 nm. The mobile phase was consisted of methanol-0.3% acetic acid solution (65∶35) with the flow rate of 0.80 mL/min, and the column temperature at 35 ℃. Results The calibration curves for Aloin and Aloeemodin were liner within 0.000 336-1.68 μg (r=0.999 96) and 0.000 608-3.04 μg (r=0.999 76), respectively. The limit of detection (LOD) was 0.05 06 ng/mL and 0.262 ng/mL, respectively. The average recovery was 95.99% and 95.80%, respectively. Conclusion The method is simple, rapid, accurate, sensitive, low cost and environment-friendly, thus it provides an effective means for assaying anthraquinones in Barbodos Aloe.
