Leaves water extract on the liver human hepatocellular carcinoma xenografts and protein expression of Bcl-2 and HSP70
10.3969/j.issn.1671-8348.2013.33.034
- VernacularTitle:艾叶水提取物对肝癌细胞的抑制作用及对Bcl-2和HSP70蛋白表达的影响
- Author:
Shutong LU
;
Shilin ZHAN
;
Jianxiong CHEN
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
HSP70 heat-shock proteins;
water extract of the leaves;
Bcl-2
- From:
Chongqing Medicine
2013;(33):4062-4063,4066
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the leaves water extract on the liver human hepatocellular carcinoma xenografts and protein expression of Bcl-2 and HSP70 .Methods From cell morphology and DNA agarose gel electrophoresis the observation leaves water extract the characteristics of the material role in human hepatoma cells ,further use of immunocytochemistry agent Western blotting analysis method oncogene Bcl-2 and tumor suppressor genes HSP70expression .Results After the processing of the the 60 mg/mL leaves water extract of liver cancer cells after 12 h ,liver cells showed severe shrinkage ,pyknosis ,highlight the cell plasma mem-brane ,immunohistochemistry and Western blotting analysis showed that during the experiment along with the oncogene Bcl-2 ex-pression weakened(P<0 .05) ,while the expression of tumor suppressor genes HSP70 had no obvious change(P<0 .05) .Conclusion Water extract of the leaves can be induced in vitro human liver cancer cell apoptosis ,and the apoptotic process may be followed by the oncogene Bcl-2 ,tumor suppressor gene expression of HSP70 relationship .
