Effect of activated PAR-1 on the expression of CXCR4 mRNA,proliferation and migration of mouse endothelial progenitor cells

VernacularTitle:PAR-1激活对小鼠内皮祖细胞CXCR4 mRNA 表达及细胞增殖与迁移的影响
Author: Jinglan DAI ; Bo PAN ; Ping GU ; Haigang MU ; Jiasi BAI
Publication Type:Journal Article
Keywords: endothelial progenitor cells; protease-activated receptor 1; CXC receptor 4; cell proliferation; migration
From: Chongqing Medicine 2013;(33):4056-4058,4061
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of protease-activated receptor 1(PAR-1) activation on the expression of CXCR4 mR-NA of mouse endothelial progenitor cells (EPCs) and proliferation ,migration of EPCs .Methods Mouse EPCs were activated by different concentration of SFLLRN (one agonist of PAR-1) ,or transfected by small interfering RNA of PAR-1 .The expressions of PAR-1 and CXCR4 mRNA of EPCs were detected by fluorescent quantitative real-time PCR .Proliferation ,migration of EPCs were detected by MTT ,Transwell chambers respectively .Results The expressions of PAR-1 and CXCR4 mRNA in SFLLRN group were higher than that in other groups(P< 0 .05) .The expression of CXCR4 mRNA was highly positive correlation with PAR-1 mRNA(r=0 .991) .The proliferation ,migration of mouse EPCs were induced by activation of PAR-1 .Conclusion Activation of PAR-1 promotes cell proliferation and migration of mouse EPCs ,this effect may be depended on CXCR4 .