Effects of TGF-β1 on contents of cytokines in the hippocampus of rats after pilocarpine induced status epilepticus
- VernacularTitle:TGF-β1对匹罗卡品癫痫大鼠海马炎性细胞因子的影响
- Author:
Huimin ZHANG
;
Yimin LIU
;
Yu WANG
- Publication Type:Journal Article
- Keywords:
TGF-β1;
IL-1β;
IL-6;
IL-18
- From:
Acta Universitatis Medicinalis Anhui
2013;(11):1316-1319,1320
- CountryChina
- Language:Chinese
-
Abstract:
Objective Via observing concentration changes of interleukin(IL)-1β, IL-6 and IL-18 in hippocampus of pilocarpine treated rats after intranasal delivery of transforming growth factor-β1(TGF-β1), to explore the possi-ble anti-epileptogenic and neuro-protective role of TGF-β1 in the epiletogenesis progression. Methods Three hours after pilocarpine induced status epilepticus, rats were treated with recombinant human TGF-β1 or the same volume of PBS by intranasal administration. Animals were executed, harvested at 24, 48, 72 h after SE individual-ly. Using the methods of the enzyme-linked immunosorbent assay (ELISA) and immunohistochemisty (IHC), the concentrations and distribution of IL-1β, IL-6, IL-18 were detected in hippocampus. Results TGF-β1 could re-duce the expression of IL-1β and IL-6 in the hippocampus of pilocarpine treated rats effectively (P<0.05 or P<0.01), however, TGF-β1 did not affect the expression of IL-18 significantly( P >0.05). Conclusion By the supplementation of intranasal TGF-β1, classical pro-inflammatory cytokine ( IL-1β, IL-6) are remarkably sup-pressed, TGF-β1 may exert antiepileptogenic and neuropretective effects.
