Comparison of MMP-9 Expression in Osteoclasts Obtained by Different Methods
- VernacularTitle:MMP-9表达在不同方法获得破骨细胞的比较研究
- Author:
Ming DU
;
Bo HE
;
Xiaochao ZHANG
;
Xueting YANG
;
Yiqin LU
;
Peng CHEN
;
Zhiqiang SHEN
- Publication Type:Journal Article
- Keywords:
Osteoclast;
Osteoclast like cells;
Mechanical method;
Inducement method;
Matrix metalloproteinase 9
- From:
Journal of Kunming Medical University
2013;(12):1-4,25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differences of protein and mRNA expression of matrix metalloproteinase 9 (MMP-9) in osteoclasts (OC) and osteoclast-like cells (OLC) obtained by mechanical and inducement methods. Methods Mechanical separation method was used to separate mature osteoclasts from long bones of SD rats aged one-day;and inducement culture method was applied to induce OLC by using RANKL (100 ng/mL) and M-CSF (100 ng/mL) . The protein expression of MMP-9 was measured by immunocytochemistry and mRNA expression of MMP-9 was assayed by in situ hybridization. Results The integral optical density (IOD) and average optical density (AOD) of positive cells in the visual field were higher in the 12-d group of inducement method as compared with the 3 d-group of mechanical method. Conclusions It is suggested that the protein and mRNA expression of MMP-9 in OLC obtained by 12 d inducement method is much high than in OC obtained by 3 d mechanical method. OLC obtained by inducement method can be applied in the study of osteoporosis.
