Content Determination of Catalpol in Zengye Chengqi Syrupus by HPLC
10.3969/j.issn.1005-5304.2014.01.025
- VernacularTitle:HPLC测定增液承气口服液中梓醇含量
- Author:
Qin WANG
;
Xiaolong YUAN
- Publication Type:Journal Article
- Keywords:
Zengye Chengqi Syrupus;
catalpol;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(1):83-84,87
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of catalpol in Zengye Chengqi Syrupus by HPLC. Methods High performance liquid chromatography was performed on an Eclipse XDB C18 column (150 mm×4.6 mm, 5 μm) with acetonitrile-0.1%phosphoric acid (1∶99) as the mobile phase at a flow rate of 1.0 mL/min, 210 nm as the detection wavelength, the temperature of column was set at 30 ℃. Results Catalpol showed good linear relationship at the range of 0.052-0.258 μg (r=0.999 9), the average recovery (n=5) was 98.23% (RSD=0.76%, n=9). Conclusion The method was accurate, reliable and specific. It can be used for the quality control and evaluation of Zengye Chengqi Syrupus.
