Experimental study on polymethylmethacrylate particles induce osteolysis
10.3969/j.issn.1671-8348.2013.34.021
- VernacularTitle:聚甲基丙烯酸甲酯颗粒诱导骨溶解实验研究
- Author:
Yan CAI
;
Qin SHI
;
Huan ZHAO
;
Qiaoli GU
- Publication Type:Journal Article
- Keywords:
methylmethacrylates;
autophage;
tartrates;
staining and labeling
- From:
Chongqing Medicine
2013;(34):4160-4161,4165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of polymethylmethacrylate(PMMA)-induced autophagy osteoclasts on bone disso-lution animal models ,and study the mechanism of PMMA particle-induced pyrophosphate osteolysis .Methods 30 8-week-old BALB / c mice were randomly divided into two groups ,sterile air with back injection on mice to form airbag and homologous skulls were implanted into experimental group mice were injected with PMMA particles ,the control group were injected with physiological saline .After 14 d ,the mice were killed ,osteoclast activation related gene (RANK / RANKL ) and autophagy morphological exami-nation of the the airbags tissue and the skull were detect .Results The tartrate-resistant enzyme staining (TRAP)-positive osteo-clasts(21 .31 ± 6 .32)s of experiment group is significantly higher than that of the control group (7 .45 ± 3 .23) ,immunohisto-chemistry showed that autophytic protein microtubule-associated protein 1 light chain 3(LC3) and Beclin 1 antibody staining score level in experimental group was significantly higher than that of control group .RT-PCR showed that the RANK mRNA level(1 .35 ± 0 .05) of experimental group was significantly increased(P< 0 .05) .Conclusion The autophagy induce by PMMA is involved in the formation of osteolysis .
