HPLC Determination of Swertiamarin in Gantaishu Capsules
10.3969/j.issn.1005-5304.2014.01.021
- VernacularTitle:HPLC测定肝泰舒胶囊中獐牙菜苦苷含量
- Author:
Shanshan GUO
;
Wenjing CAO
;
Xueyan BI
;
Qingbo ZHANG
- Publication Type:Journal Article
- Keywords:
Gantaishu capsules;
content determination;
swertiamarin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(1):69-70,71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for determining the content of swertiamarin in Gantaishu capsules. Methods Using a Waters Symmetry C18 column (4.6 mm×250 mm, 5μm), the mobile phase was acetonitrile-0.02%phosphoric acid, with flow rate of 1.0 mL/min. The detection wavelength was at 250 nm, the column temperature was room temperature. Results The content of swertiamarin showed a good linear relationship in the range of 0.373 556-2.988 448 μg, r 2=1.000 0. The average recovery was 106.81%, and RSD was 3.7%. Conclusion This method is fast, accurate and simple with good reproducibility, which can be applied to the quality control of Gantaishu capsules.
